Tabelj Oblku Vdvduvannya Dtej Forma No 305

Tabelj Oblku Vdvduvannya Dtej Forma No 305 8,9/10 7766 votes

Si Nga Sou, Dirk-Jan Kleinjan, Susan J. Rosser Institute of Quantitative Biology, Biochemistry, and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3FF, UK Correspondence: Si Nga Sou (si.sou@ed.ac.uk) Background Despite advances in Chinese hamster ovary (CHO) cell bioprocess optimisation, production of large complex proteins remains costly and high degree of variability among final products is problematic. Novel strategies that target molecular pathways for high product yield and consistency are vital. To overcome this bottleneck, we developed a CRISPR-dCas based synthetic transcription factors (sTF) system that modulates expression of endogenous mRNA and miRNA targets involved in protein transport and glycosylation. Materials and methods sTF utilises two forms of Cas9 proteins: Endonuclease inactive ‘dead’ Cas9 (dCas9) with trans-activator domain (VPR) attached and native cutting Cas9 (Fig. In Herceptin® expressing CHO-K1, we transiently expressed dCas9-VPR with sgRNAs against upstream of protein transport-related gene promoters (Napg, Rab5A & Aprc1b) for transcriptional activation, or transfecting dCas9 with sgRNAs against their promoter regions for suppression (Vamp4).

Tabelj Oblku Vdvduvannya Dtej Forma No 305

To lower galactosyltransferase (β1,4-GalT)-associated miRNA expression (cgr-miR-181d-5p, cgr-miR500 & cgr-miR501-5p), CHO cells were co-expressed with dCas9 and sgRNAs against miRNA promoters; or with native Cas9 and sgRNAs against mature miRNA sequences [1]. MRNA and miRNA levels of target genes were quantified by q-rt-PCR, protein level of β1,4-GalTs by western blot, and secreted IgG yield by IgG-ELISA. Results The dCas9 approach receives up to 60% increase in IgG expression, along with 1.2 to 2.5-fold rise in Napg, Rab5A and Aprc1b mRNA levels. While repressing Vamp4 transcription leads to a negative effect on IgG yield (Fig. Our results show positive correlation between pathways involved in protein transport and recycling, and recombinant protein (rProtein) yield. Both Cas9 and dCas9 approaches reduce miR-181d-5p, miR500 & miR501-5p by around 35-50%, this simultaneously enhances β1,4-GalT1 & 4 expression by up to 2-fold, which could be useful in future engineering of rProtein glycosylation profiles for specific function. This system also provides a platform for concurrent manipulation of multiple mRNA and miRNA with dCas9, where dCas9 expression can be further controlled via AID- or ecDFR-Degron technology [2].

FAN Farsund NO FAO Faro PT FAQ Freida River PG FAR Fargo ND US FAS Faskrudsfjord IS FAT Fresno CA US FAV Fakarava PF FAY Fayetteville NC US FBD Faizabad AF FBE Francisco Bel PR BR FBM Lubumbashi CD FCA Kalispell MT US FCB Ficksburg ZA FDE Forde NO FDF Fort-De-Franc MQ.

Conclusions Our works here present the potential of the CRISPRa/i system to easily reengineer or to study CHO cell metabolic pathways for more efficient rProtein production. The chemical inducible Cas9/dCas9 protein expression offers further control over multiple endogenous gene manipulation. Acknowledgements Authors thankfully acknowledge the Biotechnology and Biological Sciences Research Council for funding this research work. SNS thanks ESACT 2017 for providing her with the opportunity to present her work at the meeting. References 1. Tituljnie listi dlya portfolio vospitatelya rozi. Chang H, Yi B, Ma R, Zhang X, Zhao H, Xi Y. CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo.

Scientific Reports 2016. Kleinjan D, Wardrope C, Sou S, Rosser S. A Toolkit of Tunable, Degron-tagged dCas9/Cpf1 Effectors for Multi-directional Drug-inducible control of Synthetic Gene Regulation. Nat Commun 2017 (In press). Holger Laux 1, Sandrine Romand 1, Joel Tapparel 1, Sandro Nuciforo 1, Stine Buechmann-Moller 2, Guelay Dogrusoez 1, Sandra Haas 1, Benjamin Sommer 1, Edward J. Oakeley 2, Ursula Bodendorf 2 1Novartis (BTDM), Basel, 4056, Switzerland; 2Novartis (NIBR), Basel, 4056, Switzerland Correspondence: Holger Laux (holger.laux@novartis.com) Background An increasing number of biologics are entering the development pipelines of pharmaceutical companies [1].

Today, the preferred production host for therapeutic proteins is the CHO cell line. However one of the major hurdles, especially for the production of non-antibody glycoproteins, is host cell-related proteolytic degradation which can drastically impact developability and timelines of pipeline projects. Material and methods Spike-in: CHO cells were cultivated in a chemically defined culture medium at 36.5°C/10% CO 2 in shake-flasks. When the cells reached their maximum viable density, they were removed by centrifugation and the conditioned medium was collected. A model mAb was spiked into the conditioned medium and incubated at 37°C ± protease inhibitors. The amount of proteolytic degradation was analysed by western blot and LC-MS.